สำนักวิชาเภสัชศาสตร์ มหาวิทยาลัยวลัยลักษณ์ ตอกย้ำความเป็นเลิศด้านงานวิจัยและวิชาการ เผยความสำเร็จของคณาจารย์ในรอบปีงบประมาณ 2569 กับผลงานตีพิมพ์ในวารสารวิชาการระดับนานาชาติที่อยู่ในฐานข้อมูลชั้นนำ (เช่น Scopus/PubMed/Q1) สะท้อนถึงคุณภาพการเรียนการสอนและการก้าวสู่มหาวิทยาลัยวิจัยระดับแนวหน้า ในปีงบประมาณ 2569 นี้ คณาจารย์จากสำนักวิชาเภสัชศาสตร์ได้สร้างสรรค์องค์ความรู้ใหม่ผ่านงานวิจัยหลากหลายแขนง อาทิ เภสัชพฤกษศาสตร์, พฤกษเคมี, เภสัชกรรมคลินิก ซึ่งงานวิจัยเหล่านี้ไม่เพียงแต่เป็นการสร้างชื่อเสียงในระดับนานาชาติ แต่ยังมุ่งเน้นการนำไปใช้ประโยชน์จริงเพื่อแก้ไขปัญหาสุขภาพและพัฒนาอุตสาหกรรมยาในระดับภูมิภาคและประเทศ คณบดีสำนักวิชาเภสัชศาสตร์ กล่าวว่า “ความสำเร็จในครั้งนี้เกิดจากความมุ่งมั่นของคณาจารย์ที่ต้องการยกระดับมาตรฐานทางวิชาการและการวิจัยของมหาวิทยาลัยวลัยลักษณ์ให้ทัดเทียมสากล ตามนโยบายการขับเคลื่อนมหาวิทยาลัยสู่ระดับโลก (World Class University)
ผลงานวิชาการในไตรมาสที่ 1 ปีงบประมาณ 2569
- Effect of condensed tannins from black currant (Ribes nigrum) on acetylcholine-induced contractility in isolated rat intestine
Chaweewan Klongsiriwet, Watcharapong Purintrapibal, Paphon Paphochanang, Aunchalee Prammano, Nawiya Huipao, Siriphun Hiranyachattada
Abstract
| The condensed tannins (CTs) from black currant (R. nigrum) leaves were isolated as a tannin fraction to investigate their effect on isolated rat duodenal contraction induced by acetylcholine (ACh). The mechanisms of CT action were compared with those of a muscarinic receptor antagonist (atropine) and a calcium channel blocker (verapamil). Duodenal segments were fixed in organ bath, and the frequency, amplitude, and tone of contraction were recorded. The experiments were performed in seven groups, including the vehicle control, CTs (10, 50, 100, and 300 ?g/ml), atropine (1.55?10?5 M), and verapamil (10?6 M), with cumulative additions of ACh (10?8?10?4 M). The possibility that CTs might act by altering ion channel activity in intestinal epithelial cells was tested in a human colonic adenocarcinoma cell line by measuring the equivalent short-circuit current, compared with that induced by an adenylyl cyclase activator forskolin (10 ?M). It was found that CTsatlowconcentrations(10and50?g/ml)significantly decreased the frequency of ACh-induced duodenal contractions. Only the CTs at high concentration (300 ?g/ml) were able to inhibit ACh-induced amplitude and tone of contraction, similar to those of atropine, but not verapamil. Preliminary data of equivalent short-circuit currents were unaltered after the four doses of CTs, in contrast to forskolin. It is concluded that the mechanism of CT action is more likely mediated via the muscarinic receptor signaling pathway rather than through inhibition of Ca2+ channels in intestinal pacemaker and smooth musclecell membranesorepithelial cAMP-dependent Cl? secretion. |
- Turning okra into action: Sustainable nicotine transdermal plaster gels from plant-based polymers
Thipapun Plyduang, Pattwat Maneewattanapinyo, Phakorn Meksawasdichai, Chatchawat Pratin, Chaowalit Monton, Jirapornchai Suksaeree
Abstract
| Natural polymers are gaining momentum as sustainable alternatives to synthetic materials in pharmaceutical formulations. In this study, we developed a nicotine-loaded transdermal plaster gel using plant-based mucilage extracted from Abelmoschus esculentus (okra), blended with carrageenan and polyvinyl alcohol (PVA), and plasticized with glycerin. This eco-conscious formulation aims to reduce reliance on petrochemical-derived polymers while offering practical transdermal drug delivery potential. The okra mucilage yielded 2.28 ± 0.73 % of extractable powder and exhibited favorable physicochemical properties for film formation. When incorporated into the gel, variations in carrageenan and PVA content influenced color, viscosity, pH, and drying time. Films formed from these gels showed different mechanical strengths, with optimal flexibility and tensile performance achieved at a 7 % w/w mucilage level blended with both carrageenan and PVA. Thermal analysis confirmed good miscibility and thermal stability of the polymer blend, with decomposition temperatures exceeding 300 °C. In vitro studies demonstrated that nicotine release followed a first-order kinetic model, governed by formulation viscosity. Permeation through newborn pig skin followed zero-order kinetics, with steady-state flux and permeability coefficients indicating controlled delivery potential. This work demonstrates how okra-derived mucilage, a low-cost, biodegradable, and widely available natural polymer, can be harnessed for climate-conscious pharmaceutical development. The results support its use in sustainable transdermal drug delivery systems, offering a greener path forward in formulation science. |
- Advancing sustainable phytochemical extraction through design of experiments: A data-driven pathway toward low-emission natural product processing
Thipapun Plyduang, Chaowalit Monton, Pattwat Maneewattanapinyo, Jirapornchai Suksaeree
Abstract
| The growing emphasis on sustainable natural product development has sparked interest in green extraction methods that are both efficient and environmentally friendly. This review explores how Design of Experiments (DOE), particularly when applied through Design Expert® software, plays a central role in optimizing phytochemical extraction. By comparing various techniques—such as microwave-assisted, ultrasound-assisted, enzyme-assisted, and supercritical fluid extraction—the review highlights how DOE improves yield, reduces solvent use, and shortens extraction time. Case studies show that methods like central composite and Box–Behnken designs can enhance extraction efficiency by up to 500 % while maintaining compound integrity. Beyond optimization, the review emphasizes the integration of risk assessment tools (e.g., HACCP, FMEA) into DOE workflows, supporting consistency and regulatory compliance under Quality by Design (QbD) principles. It also introduces green and white analytical chemistry metrics (AGREE, GAPI, etc.) to evaluate environmental impact and method performance. Altogether, this article provides practical insights into how DOE-driven strategies can support low-emission, scalable, and regulation-ready herbal extraction within the broader shift toward Pharmacy 4.0 and climate-conscious bioprocessing. |
- Silver-Doped Mesoporous Bioactive Glass Nanoparticles With Core–Shell Architecture: Enhanced In Vitro Bioactivity, Antibacterial Efficacy, and Biocompatibility
Peerapat Thongnuek, Woranop Sukparangsi, Juntratip Jomrit, Siriporn Damrongsakkul, Patsawee Sriboonaied, Chuanliang Cao, Aruna Prasopthum
Abstract
| Orthopedic biomaterials must both prevent infection and support bone regeneration. To achieve both outcomes with bioactive glass nanoparticles (BGNs), rational nanoarchitectural design is required to decouple and tune silver-ion (Ag+) and calcium-ion (Ca2+) release, balancing antibacterial efficacy with osteogenic compatibility. Herein, we synthesized four BGN types via a modified sol–gel route: solid spheres, Ag-doped solid spheres, core–shell mesoporous BGNs, and Ag-doped core–shell mesoporous BGNs. Core–shell architectures were generated by alkaline etching followed by calcium impregnation, and silver was introduced during synthesis. Comprehensive characterization (scanning/transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy) confirmed homogeneous amorphous glass networks with spatially distinct Ca and Ag distributions in the mesoporous particles. Brunauer–Emmett–Teller analysis showed high-surface-area mesoporous BGNs (≈860 m2/g) versus solid spheres (≈17 m2/g), with markedly greater pore volume. Inductively coupled plasma mass spectrometry revealed sustained Ag+ and Ca2+ release over 21 days within sub-microgram-per-milliliter levels, consistent with the core–shell design. These physicochemical features translated into superior biological performance: Ag-doped mesoporous BGNs accelerated mineralization in simulated body fluid, exhibited strong antibacterial activity against methicillin-resistant Staphylococcus aureus and Escherichia coli, and supported the viability and osteogenic differentiation of human mesenchymal stem cells. In ovo biocompatibility testing found no vascular irritation in the Hen’s Egg Test on the Chorioallantoic Membrane (HET-CAM). Altogether, nanostructural tuning, particularly combining mesoporosity with a core–shell architecture, can optimize ion-release behavior and biological function in BGNs, advancing multifunctional nanoglasses for regenerative and antimicrobial applications. |
- MPP+induces α-synuclein oligomerization and neurite impairment by disrupting mitochondrial function and Akt signaling, with apigenin emerging as a potential neuroprotective agent
Ratchaneekorn Reudhabibadh a, Nidanut Champoochana b, Zulkiflee Kuedo c, Peungchaleoy Thammanichanon d, Pilaiwanwadee Hutamekalin d, Thunwa Binlate
Abstract
| Parkinson’s disease (PD) is a devastating neurodegenerative disorder characterized by α-synuclein accumulation and mitochondrial impairment. Mitigating α-synuclein aggregation and mitochondrial dysfunction are prominent strategies in PD treatment. Apigenin, a dietary flavonoid, exhibits anti-inflammatory neuroprotective potential in PD models. However, it is still unclear whether apigenin possesses any impact on α-synuclein aggregation and mitochondrial dysfunction. Here, the effects and underlying mechanism of apigenin were investigated using MPP+-induced PD-like pathology in SH-SY5Y cells. Our results showed that apigenin significantly ameliorated neuronal apoptosis through inhibiting Bax/caspase-3 pathway and activating Bcl-2, increased size of SH-SY5Y neurospheres, and attenuated the levels of α-synuclein oligomers in MPP+-treated cells. Furthermore, apigenin improved MPP+-induced neurite damage as indicated by increased neurite length and expression of tyrosine hydroxylase (TH), growth associated protein 43 (GAP-43) and post-synaptic density protein (PSD-95). Increased ROS levels, depleted ATP levels, and decreased mitochondrial membrane potential and p-Drp1 expression in MPP+-treated cells were alleviated by apigenin. Further studies revealed that apigenin could enhance Akt activity. By using a specific Akt inhibitor, perifosine, the effects of apigenin on preventing MPP+-triggered PD pathologies were eliminated. Together, this work demonstrated that apigenin exerted neuroprotection by mitigating α-synuclein oligomers, neurite damage, mitochondrial dysfunction and neuronal apoptosis via Akt-dependent mechanism. |
- Anti-Aging and Wound Healing Activity of Cashew Apple (Anacardium occidentale) Extract and Its Liposomal Development to Enhance Skin Permeability and Ascorbic Acid Stability
Narumon Changsan, Apichart Atipairin , Poowadon Muenraya , Pajaree Sakdiset,
Titpawan Nakpheng, Teerapol Srichana , Rutthapol Sritharadol, Neelam Balekar, Wirot Chanthorn,
Nawattakorn Nualsri, Mapasawan Lewviriyakun and Somchai Sawatdee
Abstract
| The cashew apple (Anacardium occidentale L.) is rich in antioxidant bioactive constituents that have anti-aging and wound healing properties. The objective of this study is to evaluate the biological activities of cashew apple extract (CAE) and to improve the issue involving the instability of ascorbic acid, the principal active compound, by encapsulating the extract in liposomes in order to enhance its stability and skin permeation for cosmetic applications. CAE was obtained from fresh cashew apple via ethanol maceration, solvent evaporation, and freeze-drying. Ascorbic acid content, total phenolic content (TPC), total flavonoid content (TFC), and total caffeoylquinic acid content (TCQAC) were determined. The ascorbic acid content and its tautomer in the extract were quantified using the LC-MS/MS method. Biological activities, including antioxidant, anti-tyrosinase, fibroblast collagen synthesis, cytoprotection against oxidative stress, wound healing, and cytotoxicity, were assessed. CAE was encapsulated in liposomes to enhance the stability of its inherent ascorbic acid and improve its skin in comparison to free-CAE. The CAE and liposomal-CAE were incorporated and formulated into a solution, and their physicochemical stability was assessed after storage. CAE appeared as a brown, viscous liquid with a characteristic sweet, fruity scent. Each gram of CAE contained 0.90 ± 0.05 mg of ascorbic acid, TPC, 81.40 ± 7.14 mg of gallic acid equivalents (GAE), TFC, 3.73 ± 0.30 mg of rutin equivalents (RE), and TCQAC, 4.48 ± 0.05 mg of chlorogenic acid equivalents (CGAE). CAE exhibited antioxidant properties (IC50 = 282.19 ± 11.16 and 963.66 ± 3.95 µg/mL for DPPH and ABTS assay, respectively) and weak anti-tyrosinase activity (IC50 = 4213.77 ± 138.97 µg/mL). It was non-cytotoxic to fibroblast and monocyte cells at a concentration of less than 1 mg/mL. In vitro wound healing assays demonstrated that CAE stimulated collagen production in a dose-dependent manner at CAE concentrations above 250 µg/mL. Additionally, CAE exhibited cytoprotective effects against H2O2-induced oxidative stress and did not induce inflammatory responses in immune cells. The liposomal formulation containing CAE achieved high encapsulation efficiency (79.75–84.55%) based on ascorbic acid content. In skin permeation studies, CAE-loaded liposomes demonstrated an enhancement ratio approximately two-fold greater than that of free-CAE. Stability testing over 3 months showed that the ascorbic acid content in CAE-loaded liposomes remained significantly higher than that in the free-CAE under both refrigerated and long-term conditions (30 °C/75% RH). CAE demonstrated potential anti-aging properties for improving aging skin. Liposomal incorporation markedly improved ascorbic acid stability and skin permeability. |
